---
layout: rule
id: GORULE:0000038
title: "Annotations using ISS/ISA/ISO evidence should refer to a gene product (in the 'with' column) where there exists another annotation with the same or a more granular term using experimental evidence"
type: report
fail_mode: soft
status: proposed
contact: "go-quality@lists.stanford.edu"
---
For annotations by ISO, ISA or ISS, the annotations of the gene product (col 8 info in the GAF) should be checked to ensure that an annotation exists to the same or a more granular term. For instance:

Gene Product 1 | GO:1233456 |  ISS/ISO/ISA | with: Gene Product 2
Gene Product 2 | GO:1233456 (or a descendant) | EXP (or a descendant)

Allowed evidence codes for the primary annotations: EXP, IMP, IDA, IPI, IEP, IGI, HTP, HMP, HDA, HEP, HGI.